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KMID : 0861020020170020145
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Korea Journal of Herbology
2002 Volume.17 No. 2 p.145 ~ p.149
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Quantitative Determination of 5-Hydroxymethyl-2-furaldehyde from Rehmanniae Radix Preparata according to Various Processings
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Abstract
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Objectives : Rehmanniae Radix Preparata has been used to enrich blood and supply yin for a several thousand years. We have studied on the quantity of 5-hydroxymethyl-2-furaldehyde (5-HMF) from Rehmanniae Radix Preparata at various processings and tried to develop the making method.
Methods : The Rehmanniae Radix Preparata was prepared from the steaming process with rice wine, sterilized rice wine, diluted ethanol and water each other. And the change of 5-HMF was determinated. The analysis of 5-HMF was conducted by HPLC with reversed-phase C18 column and a UV detector at 280 nm. Elution was carried out at 1.0 ml/min with 5% acetonitrile.
Results and Conclusions : From this analysis, we found out that the content of 5-HMF was increased with the number of processing times and the content of 5-HMF from Rehmanniae Radix Preparata steamed for 4 times was the same as that of Korea pharmacopoeia.
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